RNA
Part:BBa_K5377100:Design
Designed by: Jiyue Hu Group: iGEM24_ZJUT-China (2024-09-11)
Congregibacter litoralis KT71 SAM-I/IV variant riboswitch
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
When using this riboswitch in other species, it is important to ensure that the RBS (ribosome binding site) included in the riboswitch can be recognized by the species' ribosome. It has been verified that E. coli MG1655 can recognize and utilize this riboswitch.
Source
It originates from the nuclear genome of Congregibacter litoralis KT71.
References
[1]https://rnacentral.org/rna/URS0000AB2186/314285
[2]Winkler, W. C., Nahvi, A., Sudarsan, N., Barrick, J. E., & Breaker, R. R. (2003). An mRNA structure that controls gene expression by binding S-adenosylmethionine. Nature structural biology, 10(9), 701–707. https://doi.org/10.1038/nsb967