RNA

Part:BBa_K5377100:Design

Designed by: Jiyue Hu   Group: iGEM24_ZJUT-China   (2024-09-11)


Congregibacter litoralis KT71 SAM-I/IV variant riboswitch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When using this riboswitch in other species, it is important to ensure that the RBS (ribosome binding site) included in the riboswitch can be recognized by the species' ribosome. It has been verified that E. coli MG1655 can recognize and utilize this riboswitch.


Source

It originates from the nuclear genome of Congregibacter litoralis KT71.

References

[1]https://rnacentral.org/rna/URS0000AB2186/314285
[2]Winkler, W. C., Nahvi, A., Sudarsan, N., Barrick, J. E., & Breaker, R. R. (2003). An mRNA structure that controls gene expression by binding S-adenosylmethionine. Nature structural biology, 10(9), 701–707. https://doi.org/10.1038/nsb967